Lysis:Article Title: Cetuximab increases LGR5 expression and augments LGR5-targeting antibody-drug conjugate efficacy in patient-derived colorectal cancer models.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER TrypLE Express Gibco Cat#12563011 Growth factor reduced Matrigel Corning Inc. Cat#356231 Advanced DMEM/F12 Gibco Cat#12634010 EGF Gibco Cat#PMG8043 Noggin Peprotech Cat#P97466 A 83-01 StemCell Tech. .. Cat#72024 Gastrin Sigma-Aldrich Cat#G9145 B-27 Gibco Cat#17504044 N-acetylcysteine Thermo Scientific Cat#A1540914 Glutamax Gibco Cat#35050061 RIPA lysis buffer ThermoFisher Cat#89901 Halt protease and phosphatase inhibitor cocktail ThermoFisher Cat#78443 SDS Laemmli buffer Thermo Scientific CatJ60015.AD Gefitinib MedChemExpress Cat#HY-50895 Lapatinib MedChemExpress Cat#GW572016 U0126 MedChemExpress Cat#HY-12031A Trametinib Selleck Cat#GSK1120212 Cycloheximide Selleck Cat#S7418 ECL Western Blotting substrate Cytiva RPN3004 TRIzol ThermoFisher Cat#15596026 Superscript IV VILO master mix with ezDNAse enzyme ThermoFisher Cat#11766050 amfiSure qGreen master mix GenDEPOT Cat#Q5600-005 Bafilomycin A1 MedChemExpress Cat#HY-100558 MG132 MedChemExpress Cat#HY-13259 Polyethylenimine HCL Max Reagent Polysciences Cat#247651 Protein A resin Genscript Cat#L00210 pH 3 Glycine bioWORLD Cat#40121204 pH 8.5 TRIS bioWORLD Cat#40120304 N-(Amino-PEG2)-N-bis(PEG3-azide) BroadPharm Cat#BP-24369 Activa TI® Transglutaminase Ajinomoto, Modernist Pantry N/A DBCO-PEG8-VKG-CPT2 MedChemExpress N/A jetPRIME Polyplus Cat#101000015 Critical commercial assays In-Fusion Snap Assembly Takara Bio USA, Inc. Cat#638947 BCA protein assay Thermo Scientific Cat#23227 RNeasy Mini Kit Qiagen Cat#74104 Plasmid Plus Midi Kit Qiagen Cat#12943 QIAprep Spin Miniprep Kit Qiagen Cat#27104 CellTiter-Glo 2.0 assay Promega Cat#G9242 Deposited data Gene Expression profile of Cetuximab response in Colorectal Cancer Derived Xenograft Previous report 37 ; Gene Expression Omnibus GSE108277 Data from ‘‘Cetuximab increases LGR5 expression and augments LGR5-targeting antibody-drug conjugate efficacy in patientderived colorectal cancer models’’ This study; Mendeley Data http://www.doi.org/10.17632/ mmmr6nhgh7.1 (Continued on next page) e2 Cell Reports Medicine 6, 102363, October 21, 2025 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines DLD-1 ATCC Cat#CCL-221; RRID: CVCL_0248 LoVo ATCC Cat#CCL-229; RRID: CVCL_0399 RKO ATCC Cat#CRL-2577; RRID: CVCL_0504 HCT116 ATCC Cat#CCL-247; RRID: CVCL_0291 SW620 ATCC Cat#CCL-227; RRID: CVCL_0547 AGS ATCC Cat#CRL-1739; RRID: CVCL_0139 HEK293T/293T ATCC Cat#CRL-3216; RRID: CVCL_0063 LIM1215 Sigma Cat#10092301; RRID: CVCL_2574 Expi293F ThermoFisher Cat#A14527; RRID: CVCL_D615 Experimental models: Organisms/strains C57BL/6J mice The Jackson Laboratory RRID: IMSR_JAX:000664 nu/nu mice Charles River Laboratories RRID: IMSR_CRL:088 NSG mice The Jackson Laboratory RRID: IMSR_JAX:005557 Oligonucleotides EGFR siRNA CST Cat#6480 MISSION negative control siRNA Sigma Cat#SIC001 LGR5 forward primer Integrated DNA technologies N/A LGR5 reverse primer Integrated DNA technologies N/A GAPDH forward primer Integrated DNA technologies N/A GAPDH reverse primer Integrated DNA technologies N/A Recombinant DNA pCEP4 Invitrogen Cat#V04450 Human IgG1 heavy chain constant region Previous report.
Western Blot:Article Title: Cetuximab increases LGR5 expression and augments LGR5-targeting antibody-drug conjugate efficacy in patient-derived colorectal cancer models.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER TrypLE Express Gibco Cat#12563011 Growth factor reduced Matrigel Corning Inc. Cat#356231 Advanced DMEM/F12 Gibco Cat#12634010 EGF Gibco Cat#PMG8043 Noggin Peprotech Cat#P97466 A 83-01 StemCell Tech. .. Cat#72024 Gastrin Sigma-Aldrich Cat#G9145 B-27 Gibco Cat#17504044 N-acetylcysteine Thermo Scientific Cat#A1540914 Glutamax Gibco Cat#35050061 RIPA lysis buffer ThermoFisher Cat#89901 Halt protease and phosphatase inhibitor cocktail ThermoFisher Cat#78443 SDS Laemmli buffer Thermo Scientific CatJ60015.AD Gefitinib MedChemExpress Cat#HY-50895 Lapatinib MedChemExpress Cat#GW572016 U0126 MedChemExpress Cat#HY-12031A Trametinib Selleck Cat#GSK1120212 Cycloheximide Selleck Cat#S7418 ECL Western Blotting substrate Cytiva RPN3004 TRIzol ThermoFisher Cat#15596026 Superscript IV VILO master mix with ezDNAse enzyme ThermoFisher Cat#11766050 amfiSure qGreen master mix GenDEPOT Cat#Q5600-005 Bafilomycin A1 MedChemExpress Cat#HY-100558 MG132 MedChemExpress Cat#HY-13259 Polyethylenimine HCL Max Reagent Polysciences Cat#247651 Protein A resin Genscript Cat#L00210 pH 3 Glycine bioWORLD Cat#40121204 pH 8.5 TRIS bioWORLD Cat#40120304 N-(Amino-PEG2)-N-bis(PEG3-azide) BroadPharm Cat#BP-24369 Activa TI® Transglutaminase Ajinomoto, Modernist Pantry N/A DBCO-PEG8-VKG-CPT2 MedChemExpress N/A jetPRIME Polyplus Cat#101000015 Critical commercial assays In-Fusion Snap Assembly Takara Bio USA, Inc. Cat#638947 BCA protein assay Thermo Scientific Cat#23227 RNeasy Mini Kit Qiagen Cat#74104 Plasmid Plus Midi Kit Qiagen Cat#12943 QIAprep Spin Miniprep Kit Qiagen Cat#27104 CellTiter-Glo 2.0 assay Promega Cat#G9242 Deposited data Gene Expression profile of Cetuximab response in Colorectal Cancer Derived Xenograft Previous report 37 ; Gene Expression Omnibus GSE108277 Data from ‘‘Cetuximab increases LGR5 expression and augments LGR5-targeting antibody-drug conjugate efficacy in patientderived colorectal cancer models’’ This study; Mendeley Data http://www.doi.org/10.17632/ mmmr6nhgh7.1 (Continued on next page) e2 Cell Reports Medicine 6, 102363, October 21, 2025 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines DLD-1 ATCC Cat#CCL-221; RRID: CVCL_0248 LoVo ATCC Cat#CCL-229; RRID: CVCL_0399 RKO ATCC Cat#CRL-2577; RRID: CVCL_0504 HCT116 ATCC Cat#CCL-247; RRID: CVCL_0291 SW620 ATCC Cat#CCL-227; RRID: CVCL_0547 AGS ATCC Cat#CRL-1739; RRID: CVCL_0139 HEK293T/293T ATCC Cat#CRL-3216; RRID: CVCL_0063 LIM1215 Sigma Cat#10092301; RRID: CVCL_2574 Expi293F ThermoFisher Cat#A14527; RRID: CVCL_D615 Experimental models: Organisms/strains C57BL/6J mice The Jackson Laboratory RRID: IMSR_JAX:000664 nu/nu mice Charles River Laboratories RRID: IMSR_CRL:088 NSG mice The Jackson Laboratory RRID: IMSR_JAX:005557 Oligonucleotides EGFR siRNA CST Cat#6480 MISSION negative control siRNA Sigma Cat#SIC001 LGR5 forward primer Integrated DNA technologies N/A LGR5 reverse primer Integrated DNA technologies N/A GAPDH forward primer Integrated DNA technologies N/A GAPDH reverse primer Integrated DNA technologies N/A Recombinant DNA pCEP4 Invitrogen Cat#V04450 Human IgG1 heavy chain constant region Previous report.
Bicinchoninic Acid Protein Assay:Article Title: Cetuximab increases LGR5 expression and augments LGR5-targeting antibody-drug conjugate efficacy in patient-derived colorectal cancer models.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER TrypLE Express Gibco Cat#12563011 Growth factor reduced Matrigel Corning Inc. Cat#356231 Advanced DMEM/F12 Gibco Cat#12634010 EGF Gibco Cat#PMG8043 Noggin Peprotech Cat#P97466 A 83-01 StemCell Tech. .. Cat#72024 Gastrin Sigma-Aldrich Cat#G9145 B-27 Gibco Cat#17504044 N-acetylcysteine Thermo Scientific Cat#A1540914 Glutamax Gibco Cat#35050061 RIPA lysis buffer ThermoFisher Cat#89901 Halt protease and phosphatase inhibitor cocktail ThermoFisher Cat#78443 SDS Laemmli buffer Thermo Scientific CatJ60015.AD Gefitinib MedChemExpress Cat#HY-50895 Lapatinib MedChemExpress Cat#GW572016 U0126 MedChemExpress Cat#HY-12031A Trametinib Selleck Cat#GSK1120212 Cycloheximide Selleck Cat#S7418 ECL Western Blotting substrate Cytiva RPN3004 TRIzol ThermoFisher Cat#15596026 Superscript IV VILO master mix with ezDNAse enzyme ThermoFisher Cat#11766050 amfiSure qGreen master mix GenDEPOT Cat#Q5600-005 Bafilomycin A1 MedChemExpress Cat#HY-100558 MG132 MedChemExpress Cat#HY-13259 Polyethylenimine HCL Max Reagent Polysciences Cat#247651 Protein A resin Genscript Cat#L00210 pH 3 Glycine bioWORLD Cat#40121204 pH 8.5 TRIS bioWORLD Cat#40120304 N-(Amino-PEG2)-N-bis(PEG3-azide) BroadPharm Cat#BP-24369 Activa TI® Transglutaminase Ajinomoto, Modernist Pantry N/A DBCO-PEG8-VKG-CPT2 MedChemExpress N/A jetPRIME Polyplus Cat#101000015 Critical commercial assays In-Fusion Snap Assembly Takara Bio USA, Inc. Cat#638947 BCA protein assay Thermo Scientific Cat#23227 RNeasy Mini Kit Qiagen Cat#74104 Plasmid Plus Midi Kit Qiagen Cat#12943 QIAprep Spin Miniprep Kit Qiagen Cat#27104 CellTiter-Glo 2.0 assay Promega Cat#G9242 Deposited data Gene Expression profile of Cetuximab response in Colorectal Cancer Derived Xenograft Previous report 37 ; Gene Expression Omnibus GSE108277 Data from ‘‘Cetuximab increases LGR5 expression and augments LGR5-targeting antibody-drug conjugate efficacy in patientderived colorectal cancer models’’ This study; Mendeley Data http://www.doi.org/10.17632/ mmmr6nhgh7.1 (Continued on next page) e2 Cell Reports Medicine 6, 102363, October 21, 2025 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines DLD-1 ATCC Cat#CCL-221; RRID: CVCL_0248 LoVo ATCC Cat#CCL-229; RRID: CVCL_0399 RKO ATCC Cat#CRL-2577; RRID: CVCL_0504 HCT116 ATCC Cat#CCL-247; RRID: CVCL_0291 SW620 ATCC Cat#CCL-227; RRID: CVCL_0547 AGS ATCC Cat#CRL-1739; RRID: CVCL_0139 HEK293T/293T ATCC Cat#CRL-3216; RRID: CVCL_0063 LIM1215 Sigma Cat#10092301; RRID: CVCL_2574 Expi293F ThermoFisher Cat#A14527; RRID: CVCL_D615 Experimental models: Organisms/strains C57BL/6J mice The Jackson Laboratory RRID: IMSR_JAX:000664 nu/nu mice Charles River Laboratories RRID: IMSR_CRL:088 NSG mice The Jackson Laboratory RRID: IMSR_JAX:005557 Oligonucleotides EGFR siRNA CST Cat#6480 MISSION negative control siRNA Sigma Cat#SIC001 LGR5 forward primer Integrated DNA technologies N/A LGR5 reverse primer Integrated DNA technologies N/A GAPDH forward primer Integrated DNA technologies N/A GAPDH reverse primer Integrated DNA technologies N/A Recombinant DNA pCEP4 Invitrogen Cat#V04450 Human IgG1 heavy chain constant region Previous report.
Plasmid Preparation:Article Title: Cetuximab increases LGR5 expression and augments LGR5-targeting antibody-drug conjugate efficacy in patient-derived colorectal cancer models.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER TrypLE Express Gibco Cat#12563011 Growth factor reduced Matrigel Corning Inc. Cat#356231 Advanced DMEM/F12 Gibco Cat#12634010 EGF Gibco Cat#PMG8043 Noggin Peprotech Cat#P97466 A 83-01 StemCell Tech. .. Cat#72024 Gastrin Sigma-Aldrich Cat#G9145 B-27 Gibco Cat#17504044 N-acetylcysteine Thermo Scientific Cat#A1540914 Glutamax Gibco Cat#35050061 RIPA lysis buffer ThermoFisher Cat#89901 Halt protease and phosphatase inhibitor cocktail ThermoFisher Cat#78443 SDS Laemmli buffer Thermo Scientific CatJ60015.AD Gefitinib MedChemExpress Cat#HY-50895 Lapatinib MedChemExpress Cat#GW572016 U0126 MedChemExpress Cat#HY-12031A Trametinib Selleck Cat#GSK1120212 Cycloheximide Selleck Cat#S7418 ECL Western Blotting substrate Cytiva RPN3004 TRIzol ThermoFisher Cat#15596026 Superscript IV VILO master mix with ezDNAse enzyme ThermoFisher Cat#11766050 amfiSure qGreen master mix GenDEPOT Cat#Q5600-005 Bafilomycin A1 MedChemExpress Cat#HY-100558 MG132 MedChemExpress Cat#HY-13259 Polyethylenimine HCL Max Reagent Polysciences Cat#247651 Protein A resin Genscript Cat#L00210 pH 3 Glycine bioWORLD Cat#40121204 pH 8.5 TRIS bioWORLD Cat#40120304 N-(Amino-PEG2)-N-bis(PEG3-azide) BroadPharm Cat#BP-24369 Activa TI® Transglutaminase Ajinomoto, Modernist Pantry N/A DBCO-PEG8-VKG-CPT2 MedChemExpress N/A jetPRIME Polyplus Cat#101000015 Critical commercial assays In-Fusion Snap Assembly Takara Bio USA, Inc. Cat#638947 BCA protein assay Thermo Scientific Cat#23227 RNeasy Mini Kit Qiagen Cat#74104 Plasmid Plus Midi Kit Qiagen Cat#12943 QIAprep Spin Miniprep Kit Qiagen Cat#27104 CellTiter-Glo 2.0 assay Promega Cat#G9242 Deposited data Gene Expression profile of Cetuximab response in Colorectal Cancer Derived Xenograft Previous report 37 ; Gene Expression Omnibus GSE108277 Data from ‘‘Cetuximab increases LGR5 expression and augments LGR5-targeting antibody-drug conjugate efficacy in patientderived colorectal cancer models’’ This study; Mendeley Data http://www.doi.org/10.17632/ mmmr6nhgh7.1 (Continued on next page) e2 Cell Reports Medicine 6, 102363, October 21, 2025 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines DLD-1 ATCC Cat#CCL-221; RRID: CVCL_0248 LoVo ATCC Cat#CCL-229; RRID: CVCL_0399 RKO ATCC Cat#CRL-2577; RRID: CVCL_0504 HCT116 ATCC Cat#CCL-247; RRID: CVCL_0291 SW620 ATCC Cat#CCL-227; RRID: CVCL_0547 AGS ATCC Cat#CRL-1739; RRID: CVCL_0139 HEK293T/293T ATCC Cat#CRL-3216; RRID: CVCL_0063 LIM1215 Sigma Cat#10092301; RRID: CVCL_2574 Expi293F ThermoFisher Cat#A14527; RRID: CVCL_D615 Experimental models: Organisms/strains C57BL/6J mice The Jackson Laboratory RRID: IMSR_JAX:000664 nu/nu mice Charles River Laboratories RRID: IMSR_CRL:088 NSG mice The Jackson Laboratory RRID: IMSR_JAX:005557 Oligonucleotides EGFR siRNA CST Cat#6480 MISSION negative control siRNA Sigma Cat#SIC001 LGR5 forward primer Integrated DNA technologies N/A LGR5 reverse primer Integrated DNA technologies N/A GAPDH forward primer Integrated DNA technologies N/A GAPDH reverse primer Integrated DNA technologies N/A Recombinant DNA pCEP4 Invitrogen Cat#V04450 Human IgG1 heavy chain constant region Previous report.
Gene Expression:Article Title: Cetuximab increases LGR5 expression and augments LGR5-targeting antibody-drug conjugate efficacy in patient-derived colorectal cancer models.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER TrypLE Express Gibco Cat#12563011 Growth factor reduced Matrigel Corning Inc. Cat#356231 Advanced DMEM/F12 Gibco Cat#12634010 EGF Gibco Cat#PMG8043 Noggin Peprotech Cat#P97466 A 83-01 StemCell Tech. .. Cat#72024 Gastrin Sigma-Aldrich Cat#G9145 B-27 Gibco Cat#17504044 N-acetylcysteine Thermo Scientific Cat#A1540914 Glutamax Gibco Cat#35050061 RIPA lysis buffer ThermoFisher Cat#89901 Halt protease and phosphatase inhibitor cocktail ThermoFisher Cat#78443 SDS Laemmli buffer Thermo Scientific CatJ60015.AD Gefitinib MedChemExpress Cat#HY-50895 Lapatinib MedChemExpress Cat#GW572016 U0126 MedChemExpress Cat#HY-12031A Trametinib Selleck Cat#GSK1120212 Cycloheximide Selleck Cat#S7418 ECL Western Blotting substrate Cytiva RPN3004 TRIzol ThermoFisher Cat#15596026 Superscript IV VILO master mix with ezDNAse enzyme ThermoFisher Cat#11766050 amfiSure qGreen master mix GenDEPOT Cat#Q5600-005 Bafilomycin A1 MedChemExpress Cat#HY-100558 MG132 MedChemExpress Cat#HY-13259 Polyethylenimine HCL Max Reagent Polysciences Cat#247651 Protein A resin Genscript Cat#L00210 pH 3 Glycine bioWORLD Cat#40121204 pH 8.5 TRIS bioWORLD Cat#40120304 N-(Amino-PEG2)-N-bis(PEG3-azide) BroadPharm Cat#BP-24369 Activa TI® Transglutaminase Ajinomoto, Modernist Pantry N/A DBCO-PEG8-VKG-CPT2 MedChemExpress N/A jetPRIME Polyplus Cat#101000015 Critical commercial assays In-Fusion Snap Assembly Takara Bio USA, Inc. Cat#638947 BCA protein assay Thermo Scientific Cat#23227 RNeasy Mini Kit Qiagen Cat#74104 Plasmid Plus Midi Kit Qiagen Cat#12943 QIAprep Spin Miniprep Kit Qiagen Cat#27104 CellTiter-Glo 2.0 assay Promega Cat#G9242 Deposited data Gene Expression profile of Cetuximab response in Colorectal Cancer Derived Xenograft Previous report 37 ; Gene Expression Omnibus GSE108277 Data from ‘‘Cetuximab increases LGR5 expression and augments LGR5-targeting antibody-drug conjugate efficacy in patientderived colorectal cancer models’’ This study; Mendeley Data http://www.doi.org/10.17632/ mmmr6nhgh7.1 (Continued on next page) e2 Cell Reports Medicine 6, 102363, October 21, 2025 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines DLD-1 ATCC Cat#CCL-221; RRID: CVCL_0248 LoVo ATCC Cat#CCL-229; RRID: CVCL_0399 RKO ATCC Cat#CRL-2577; RRID: CVCL_0504 HCT116 ATCC Cat#CCL-247; RRID: CVCL_0291 SW620 ATCC Cat#CCL-227; RRID: CVCL_0547 AGS ATCC Cat#CRL-1739; RRID: CVCL_0139 HEK293T/293T ATCC Cat#CRL-3216; RRID: CVCL_0063 LIM1215 Sigma Cat#10092301; RRID: CVCL_2574 Expi293F ThermoFisher Cat#A14527; RRID: CVCL_D615 Experimental models: Organisms/strains C57BL/6J mice The Jackson Laboratory RRID: IMSR_JAX:000664 nu/nu mice Charles River Laboratories RRID: IMSR_CRL:088 NSG mice The Jackson Laboratory RRID: IMSR_JAX:005557 Oligonucleotides EGFR siRNA CST Cat#6480 MISSION negative control siRNA Sigma Cat#SIC001 LGR5 forward primer Integrated DNA technologies N/A LGR5 reverse primer Integrated DNA technologies N/A GAPDH forward primer Integrated DNA technologies N/A GAPDH reverse primer Integrated DNA technologies N/A Recombinant DNA pCEP4 Invitrogen Cat#V04450 Human IgG1 heavy chain constant region Previous report.
Derivative Assay:Article Title: Cetuximab increases LGR5 expression and augments LGR5-targeting antibody-drug conjugate efficacy in patient-derived colorectal cancer models.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER TrypLE Express Gibco Cat#12563011 Growth factor reduced Matrigel Corning Inc. Cat#356231 Advanced DMEM/F12 Gibco Cat#12634010 EGF Gibco Cat#PMG8043 Noggin Peprotech Cat#P97466 A 83-01 StemCell Tech. .. Cat#72024 Gastrin Sigma-Aldrich Cat#G9145 B-27 Gibco Cat#17504044 N-acetylcysteine Thermo Scientific Cat#A1540914 Glutamax Gibco Cat#35050061 RIPA lysis buffer ThermoFisher Cat#89901 Halt protease and phosphatase inhibitor cocktail ThermoFisher Cat#78443 SDS Laemmli buffer Thermo Scientific CatJ60015.AD Gefitinib MedChemExpress Cat#HY-50895 Lapatinib MedChemExpress Cat#GW572016 U0126 MedChemExpress Cat#HY-12031A Trametinib Selleck Cat#GSK1120212 Cycloheximide Selleck Cat#S7418 ECL Western Blotting substrate Cytiva RPN3004 TRIzol ThermoFisher Cat#15596026 Superscript IV VILO master mix with ezDNAse enzyme ThermoFisher Cat#11766050 amfiSure qGreen master mix GenDEPOT Cat#Q5600-005 Bafilomycin A1 MedChemExpress Cat#HY-100558 MG132 MedChemExpress Cat#HY-13259 Polyethylenimine HCL Max Reagent Polysciences Cat#247651 Protein A resin Genscript Cat#L00210 pH 3 Glycine bioWORLD Cat#40121204 pH 8.5 TRIS bioWORLD Cat#40120304 N-(Amino-PEG2)-N-bis(PEG3-azide) BroadPharm Cat#BP-24369 Activa TI® Transglutaminase Ajinomoto, Modernist Pantry N/A DBCO-PEG8-VKG-CPT2 MedChemExpress N/A jetPRIME Polyplus Cat#101000015 Critical commercial assays In-Fusion Snap Assembly Takara Bio USA, Inc. Cat#638947 BCA protein assay Thermo Scientific Cat#23227 RNeasy Mini Kit Qiagen Cat#74104 Plasmid Plus Midi Kit Qiagen Cat#12943 QIAprep Spin Miniprep Kit Qiagen Cat#27104 CellTiter-Glo 2.0 assay Promega Cat#G9242 Deposited data Gene Expression profile of Cetuximab response in Colorectal Cancer Derived Xenograft Previous report 37 ; Gene Expression Omnibus GSE108277 Data from ‘‘Cetuximab increases LGR5 expression and augments LGR5-targeting antibody-drug conjugate efficacy in patientderived colorectal cancer models’’ This study; Mendeley Data http://www.doi.org/10.17632/ mmmr6nhgh7.1 (Continued on next page) e2 Cell Reports Medicine 6, 102363, October 21, 2025 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines DLD-1 ATCC Cat#CCL-221; RRID: CVCL_0248 LoVo ATCC Cat#CCL-229; RRID: CVCL_0399 RKO ATCC Cat#CRL-2577; RRID: CVCL_0504 HCT116 ATCC Cat#CCL-247; RRID: CVCL_0291 SW620 ATCC Cat#CCL-227; RRID: CVCL_0547 AGS ATCC Cat#CRL-1739; RRID: CVCL_0139 HEK293T/293T ATCC Cat#CRL-3216; RRID: CVCL_0063 LIM1215 Sigma Cat#10092301; RRID: CVCL_2574 Expi293F ThermoFisher Cat#A14527; RRID: CVCL_D615 Experimental models: Organisms/strains C57BL/6J mice The Jackson Laboratory RRID: IMSR_JAX:000664 nu/nu mice Charles River Laboratories RRID: IMSR_CRL:088 NSG mice The Jackson Laboratory RRID: IMSR_JAX:005557 Oligonucleotides EGFR siRNA CST Cat#6480 MISSION negative control siRNA Sigma Cat#SIC001 LGR5 forward primer Integrated DNA technologies N/A LGR5 reverse primer Integrated DNA technologies N/A GAPDH forward primer Integrated DNA technologies N/A GAPDH reverse primer Integrated DNA technologies N/A Recombinant DNA pCEP4 Invitrogen Cat#V04450 Human IgG1 heavy chain constant region Previous report.
Expressing:Article Title: Cetuximab increases LGR5 expression and augments LGR5-targeting antibody-drug conjugate efficacy in patient-derived colorectal cancer models.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER TrypLE Express Gibco Cat#12563011 Growth factor reduced Matrigel Corning Inc. Cat#356231 Advanced DMEM/F12 Gibco Cat#12634010 EGF Gibco Cat#PMG8043 Noggin Peprotech Cat#P97466 A 83-01 StemCell Tech. .. Cat#72024 Gastrin Sigma-Aldrich Cat#G9145 B-27 Gibco Cat#17504044 N-acetylcysteine Thermo Scientific Cat#A1540914 Glutamax Gibco Cat#35050061 RIPA lysis buffer ThermoFisher Cat#89901 Halt protease and phosphatase inhibitor cocktail ThermoFisher Cat#78443 SDS Laemmli buffer Thermo Scientific CatJ60015.AD Gefitinib MedChemExpress Cat#HY-50895 Lapatinib MedChemExpress Cat#GW572016 U0126 MedChemExpress Cat#HY-12031A Trametinib Selleck Cat#GSK1120212 Cycloheximide Selleck Cat#S7418 ECL Western Blotting substrate Cytiva RPN3004 TRIzol ThermoFisher Cat#15596026 Superscript IV VILO master mix with ezDNAse enzyme ThermoFisher Cat#11766050 amfiSure qGreen master mix GenDEPOT Cat#Q5600-005 Bafilomycin A1 MedChemExpress Cat#HY-100558 MG132 MedChemExpress Cat#HY-13259 Polyethylenimine HCL Max Reagent Polysciences Cat#247651 Protein A resin Genscript Cat#L00210 pH 3 Glycine bioWORLD Cat#40121204 pH 8.5 TRIS bioWORLD Cat#40120304 N-(Amino-PEG2)-N-bis(PEG3-azide) BroadPharm Cat#BP-24369 Activa TI® Transglutaminase Ajinomoto, Modernist Pantry N/A DBCO-PEG8-VKG-CPT2 MedChemExpress N/A jetPRIME Polyplus Cat#101000015 Critical commercial assays In-Fusion Snap Assembly Takara Bio USA, Inc. Cat#638947 BCA protein assay Thermo Scientific Cat#23227 RNeasy Mini Kit Qiagen Cat#74104 Plasmid Plus Midi Kit Qiagen Cat#12943 QIAprep Spin Miniprep Kit Qiagen Cat#27104 CellTiter-Glo 2.0 assay Promega Cat#G9242 Deposited data Gene Expression profile of Cetuximab response in Colorectal Cancer Derived Xenograft Previous report 37 ; Gene Expression Omnibus GSE108277 Data from ‘‘Cetuximab increases LGR5 expression and augments LGR5-targeting antibody-drug conjugate efficacy in patientderived colorectal cancer models’’ This study; Mendeley Data http://www.doi.org/10.17632/ mmmr6nhgh7.1 (Continued on next page) e2 Cell Reports Medicine 6, 102363, October 21, 2025 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines DLD-1 ATCC Cat#CCL-221; RRID: CVCL_0248 LoVo ATCC Cat#CCL-229; RRID: CVCL_0399 RKO ATCC Cat#CRL-2577; RRID: CVCL_0504 HCT116 ATCC Cat#CCL-247; RRID: CVCL_0291 SW620 ATCC Cat#CCL-227; RRID: CVCL_0547 AGS ATCC Cat#CRL-1739; RRID: CVCL_0139 HEK293T/293T ATCC Cat#CRL-3216; RRID: CVCL_0063 LIM1215 Sigma Cat#10092301; RRID: CVCL_2574 Expi293F ThermoFisher Cat#A14527; RRID: CVCL_D615 Experimental models: Organisms/strains C57BL/6J mice The Jackson Laboratory RRID: IMSR_JAX:000664 nu/nu mice Charles River Laboratories RRID: IMSR_CRL:088 NSG mice The Jackson Laboratory RRID: IMSR_JAX:005557 Oligonucleotides EGFR siRNA CST Cat#6480 MISSION negative control siRNA Sigma Cat#SIC001 LGR5 forward primer Integrated DNA technologies N/A LGR5 reverse primer Integrated DNA technologies N/A GAPDH forward primer Integrated DNA technologies N/A GAPDH reverse primer Integrated DNA technologies N/A Recombinant DNA pCEP4 Invitrogen Cat#V04450 Human IgG1 heavy chain constant region Previous report.
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